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Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 493-502 doi: 10.1007/s11684-022-0946-x

Abstract: Anaplastic lymphoma kinase (<i>ALKi>) is the most common fusion gene involved in non-small cell lungIn this work, we investigated whether the variant allele frequencies (VAFs) of <i>ALKi> fusions canALKi> positive.ALKi> subclonality with crizotinib efficacy was assessed.Results suggest that the <i>ALKi> VAF determined by hybrid capture-based NGS is probably unreliable

Keywords： <i>ALKi> fusion     next-generation sequencing     fluorescence <i>in situi> hybridization     immunohistochemistry    

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Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence<i>in situi> Hybridization Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Engineering 2022, Volume 9, Issue 2,   Pages 61-66 doi: 10.1016/j.eng.2021.05.012

Abstract: harvested from an anaerobic sludge blanket reactor treating phenolic wastewater was investigated using fluorescencein situ hybridization (FISH) and clone library construction.

Keywords： Cryptanaerobacter     Fluorescence     in situ     hybridization     Anaerobic phenol degradation     Syntrophorhabdaceae    

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 48-58 doi: 10.15302/J-FASE-2016114

Abstract: Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

Keywords： <i>Puccinia striiformisi> f. sp. <i>triticii>     selfing     heterozygosity     virulence inheritance    

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hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 92-102 doi: 10.1007/s11705-021-2063-9

Abstract: Herein, we constructed an <i>in situi> hydrophobic environment-triggering reactive fluorescence probeThe fluorophore was 2-styrene-1<i>Hi>-benzo[<i>di>]imidazole, and malononitrile was introduced asThus, MBI-CN can be hydrolyzed to generate MBI-CHO in an <i>in situi> hydrophobic environment withMeanwhile, MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm

Keywords： hydrolysis reaction     mitochondrial DNA damage     <i>in situi> hydrophobic environment trigger     fluorescence    

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SWIR Fluorescence Imaging <i>In Vivoi> Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Article

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

Engineering doi: 10.1016/j.eng.2023.05.010

Abstract: Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivoSWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscleThen, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the

Keywords： <i>In vivoi>     Short-wave infrared     Skeletal muscle     Macrophage     Regeneration    

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Hormesis-like growth and photosynthetic physiology of marine diatom Bohlin exposed to polystyrene microplastics

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 1,   Pages 2-2 doi: 10.1007/s11783-021-1436-0

Abstract:

• Polystyrene microplastic caused hormesis-like effects in <i>Phaeodactylumtricornutumi>.

Keywords： Microplastics     Marine microalgae     <i>Phaeodactylum tricornutumi> Bohlin     Growth     Photosynthetic pigments     Chlorophyll fluorescence parameters    

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Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing

Grant Perry, Fernando Cortezon-Tamarit, Sofia I. Pascu

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 1,   Pages 4-18 doi: 10.1007/s11705-019-1846-8

Abstract: Prostate cancer has a high incidence in men and remains the second cause of mortality due to cancer. As the development of the disease is greatly correlated to age, the identification of novel detection methods reliable, efficient, and cost effective is a matter of significant importance in the ageing population of western societies. The detection of the prostate specific antigen (PSA) in blood samples has been the preferred method for the detection and monitoring of prostate cancer over the past decades. Despite the indications against its use in massive population screening, PSA still remains the best studied biomarker for prostate cancer and the detection of its different forms and incorporation in multiplexed designs with other biomarkers still remains a highly valuable indicator in the theranostics of prostate cancer. The latest developments in the use of nanomaterials towards the construction of PSA biosensors are reviewed hereby. The incorporation of gold nanoparticles, silica nanoparticles and graphene nanostructures to biosensing devices has represented a big leap forward in terms of sensitivity, stability and miniaturization. Both electrochemical and optical detection methods will be reviewed herein for the detection of PSA.

Keywords： biosensing     immunosensor     PSA     prostate cancer     fluorescence    

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Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review Review

Lei Cui,Yongkang Ren,Timothy D. Murray,Wenze Yan,Qing Guo,Yuqi Niu,Yu Sun,Hongjie Li

Engineering 2018, Volume 4, Issue 4,   Pages 507-513 doi: 10.1016/j.eng.2018.07.003

Abstract:

Wheatgrasses (Thinopyrum spp.), which are relatives of wheat (Triticum aestivum L.), have a perennial growth habit and offer resistance to a diversity of biotic and abiotic stresses, making them useful in wheat improvement. Many of these desirable traits from Thinopyrum spp. have been used to develop wheat cultivars by introgression breeding. The perennial growth habit of wheatgrasses inherits as a complex quantitative trait that is controlled by many unknown genes. Previous studies have indicated that Thinopyrum spp. are able to hybridize with wheat and produce viable/stable amphiploids or partial amphiploids. Meanwhile, efforts have been made to develop perennial wheat by domestication of Thinopyrum spp. The most promising perennial wheat–Thinopyrum lines can be used as grain and/or forage crops, which combine the desirable traits of both parents. The wheat–Thinopyrum lines can adapt to diverse agricultural systems. This paper summarizes the development of perennial wheat based on Thinopyrum, and the genetic aspects, breeding methods, and perspectives of wheat–Thinopyrum hybrids.

Keywords： <i>Thinopyrumi>     Wheatgrass     Perennial     <i>Triticum aestivumi>    

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Formation of disinfection byproducts from accumulated soluble products of oleaginous microalga after chlorination

Yu Liu, Qiao Zhang, Yu Hong

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 6, doi: 10.1007/s11783-017-0938-2

Abstract: The predominant DBP precursors from SAP were identified with the 3D excitation-emission matrix fluorescenceAfter chlorination, a significant reduction was observed in the fluorescence intensity of five specificfluorescence regions, particularly aromatic proteins and soluble microbial by-product-like regions,

Keywords： <i>Chlorellai> sp.HQ     Chlorination     Disinfection byproducts     Fluorescence spectroscopy     Soluble algal products    

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Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

Frontiers of Environmental Science & Engineering 2013, Volume 7, Issue 4,   Pages 616-624 doi: 10.1007/s11783-013-0505-4

Abstract: The short-term effect of anaerobic reaction time (AnRT) (i.e., 90, 120 and 150 min) on the denitrifyingThe fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated

Keywords： Denitrifying phosphorus removal     anaerobic reaction time     nitrous oxide     polyhydroxyalkanoate     free nitrous acid     fluorescencein-situ hybridization    

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Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 121-122 doi: 10.15302/J-FASE-2017142

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Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Frontiers of Structural and Civil Engineering 2019, Volume 13, Issue 6,   Pages 1338-1349 doi: 10.1007/s17009-019-0558-2

Abstract: main concentration being on energy absorption characteristics of concrete and the efficacy of fiber hybridization

Keywords： hybrid fiber-reinforced concrete     synergy     toughness     steel fibers     polypropylene fibers    

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Synthesis and fluorescence behavior of 2,5-diphenyl-1,3,4-oxadiazole-containing bismaleimides and bissuccinimides

Xin ZHANG, Zichen LI

Frontiers of Chemical Science and Engineering 2013, Volume 7, Issue 4,   Pages 381-387 doi: 10.1007/s11705-013-1359-9

Abstract: Almost no or very weak fluorescence was observed for these bismaleimides, however, the bissuccinimidesshow a strong fluorescence.by UV-vis absorption and fluorescence emission spectroscopy.-Bissuccinimide displays increasing fluorescence quantum yields with red shifts of 22–24 nm, comparedPolymerizable C=C bonds play a key role in the intramolecular fluorescence quenching.

Keywords： fluorescence     bismaleimide     oxadiazole     succinimide    

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Dual enzyme activated fluorescein based fluorescent probe

Maria L. Odyniec, Jordan E. Gardiner, Adam C. Sedgwick, Xiao-Peng He, Steven D. Bull, Tony D. James

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 1,   Pages 117-121 doi: 10.1007/s11705-018-1785-9

Abstract: This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.

Keywords： chemosensors     dual-activation     GOx     fluorescence     <i>βi>-glucosidase     molecular logic    

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Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence

ZHU Xiaoqin, QIAN Ying, LU Zhifeng

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 381-384 doi: 10.1007/s11705-007-0069-6

Abstract: phenyl]-1,3,4-oxadiazoles that exhibit strong two-photon absorption and enhanced two-photon excited fluorescence-conjugated bridging unit can enhance the two-photon absorption and two-photon fluorescence.

Keywords： 2-arylvinyl     trans-vinylene character     effective     fluorescence     push-core-pull-core-push    

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Title Author Date Type Operation

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Journal Article

Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence<i>in situi> Hybridization

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Journal Article

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Journal Article

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Journal Article

SWIR Fluorescence Imaging <i>In Vivoi> Monitoring and Evaluating Implanted M2 Macrophages in Skeletal

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

Journal Article

Hormesis-like growth and photosynthetic physiology of marine diatom Bohlin exposed to polystyrene microplastics

Journal Article

Detection and monitoring prostate specific antigen using nanotechnology approaches to biosensing

Grant Perry, Fernando Cortezon-Tamarit, Sofia I. Pascu

Journal Article

Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review

Lei Cui,Yongkang Ren,Timothy D. Murray,Wenze Yan,Qing Guo,Yuqi Niu,Yu Sun,Hongjie Li

Journal Article

Formation of disinfection byproducts from accumulated soluble products of oleaginous microalga after chlorination

Yu Liu, Qiao Zhang, Yu Hong

Journal Article

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate- accumulating organisms linked to phosphorus removal and N

Gang GUO, Yayi WANG, Chong WANG, Hong WANG, Mianli PAN, Shaowei CHEN

Journal Article

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

Journal Article

Effect of fiber hybridization on energy absorption and synergy in concrete

Ahmadreza RAMEZANI, Mohammad Reza ESFAHANI

Journal Article

Synthesis and fluorescence behavior of 2,5-diphenyl-1,3,4-oxadiazole-containing bismaleimides and bissuccinimides

Xin ZHANG, Zichen LI

Journal Article

Dual enzyme activated fluorescein based fluorescent probe

Maria L. Odyniec, Jordan E. Gardiner, Adam C. Sedgwick, Xiao-Peng He, Steven D. Bull, Tony D. James

Journal Article

Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence

ZHU Xiaoqin, QIAN Ying, LU Zhifeng

Journal Article